Reliant RNA分析試劑盒
選擇Reliant RNA分析試劑盒,成分齊全! ------生產(chǎn)商:Lonza(原Cambrex)
Kit contents:(試劑盒成分)
㈠ Full box of Reliant Precast RNA Gels (Reliant預(yù)制RNA凝膠)
㈡ Sample buffer - Glyoxal or Formaldehyde (樣品緩沖液—甲醛或乙二醛)
㈢ RNA Marker (RNA標(biāo)準(zhǔn)物)
㈣ AccuGENE 10X MOPS Buffer (AccuGENE電泳液)
㈤ GelStar Nucleic Acid Stain (核酸染料)
其中:㈠Reliant Precast RNA Gels(Reliant預(yù)制RNA凝膠)
Reliant預(yù)制RNA凝膠是即用型的無RNase瓊脂糖凝膠成品,適用于多種不同的用途,如Northern blotting和RNA轉(zhuǎn)錄本的分析等。使用1.25% SeaKem Gold瓊脂糖和1X MOPS緩沖液配制(不含變性劑)
Specifications(規(guī)格)
①Gel Dimensions(凝膠尺寸): 6.0 x 9.5 cm
②Gel Tray Dimensions(凝膠盤尺寸): 6.8 x 102 cm
③Thickness(凝膠厚度): <5.5 mm
④Well volume(孔體積): <20 祃
⑤Storage conditions(儲(chǔ)存條件): 18-26度, 12 months
⑥Chamber compatibility(電泳槽兼容性): Fits most common mini chambers
(適合大多數(shù)常規(guī)小型電泳系統(tǒng))
附操作說明———Electrophoresis:(電泳)
1. Dilute the 10X MOPS buffer to 1X with RNase-free water.
2. Peel the cover from the Reliant? Gel tray.
3. Remove the protective strips from the tape on the bottom of the tray,
and press the tray onto the electrophoresis chamber platform.
4. Pour the 1X MOPS buffer into the electrophoresis
chamber, covering the gel tray flange to a depth of 5 mm.
5. Add 1 μl of gel loading buffer to each sample and mix.
6. Load up to 20 μl of RNA sample to gel.
7. Electrophorese for 2 hours at a field strength of 3.5 V/cm
(cm = interelectrode distance).
貨 號(hào) |
產(chǎn) 品 說 明 |
備 注 |
54975 |
Reliant RNA Analysis Kit,8 well formaldehyde buff |
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54976 |
Reliant RNA Analysis Kit,20 well formaldehyde buff |
|
54977 |
Reliant RNA Analysis Kit,8 well glyoxal buffer |
|
54978 |
Reliant RNA Analysis Kit,20 well glyoxal buffer |
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